Figure 6.

GFP-Sed5p moves to the ER in sec12 cells. (A) Single optical sections showing GFP-Sed5p in sec12 cells at 20°C and at 37°C (30 min after initiation of warming). Arrows indicate nuclear envelope fluorescence (compare BiP staining in Figure 1). (B) Control showing GFP-Sed5p in SEC⁺ cells. (C) Time course of movement of GFP-Sed5p in sec12 cells. Images (single optical sections) were taken at the times indicated (min), zero being the point at which heating was initiated. Just before the −1 time point, the left half of the field was photobleached. Note that the loss of punctate fluorescence and the emergence of the ER pattern in the unbleached cells occurred more rapidly than the reappearance of fluorescence in the bleached cells.