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Dispersal of the early-Golgi marker Anp1p. (A) Cells of the indicated genotypes expressing epitope-tagged Sed5p were shifted from 20°C to 37°C for 15 min, fixed, and stained with appropriate antibodies to detect the tagged Sed5p. Arrows indicate nuclear envelope (i.e., ER) staining. (B) Cells of the indicated genotypes were incubated at 20°C or shifted to 37°C for 15 min, fixed, and stained with anti-Anp1p antibodies. Single confocal sections are shown in each case.
