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Subcellular fractionation of Golgi proteins in sec12 cells. SEC+ and sec12 strains expressing either GFP-Sft2p or a myc-tagged version of Emp47p (Lewis and Pelham, 1996) were incubated at 37°C for 90 min, and medium-speed (p13) and high-speed (p100) pellet fractions were prepared. These were immunoblotted with appropriate antibodies as indicated.
