Table 3.
Suppression analysis among cofactor homologs
| Deleted gene | Gene on plasmid
|
|||
|---|---|---|---|---|
| Vector | alp11+ | alp21+ | alp1+ | |
| Δalp11 | − | + | + | + |
| Δalp21 | − | − | + | + |
| Δalp1 | − | − | − | + |
Heterozygous diploids (ME1, PR7, and PR8; see Table 1) were transformed with a vector (pREP1) or multicopy plasmids containing individual genes. Diploid transformants were allowed to sporulate, and free spores were spread on minimal plates supplemented with histidine and adenine to select viable Leu+Ura+ haploid colonies. (+) We obtained Leu+Ura+ haploid cells that contain the deleted gene carrying the indicated plasmids; (−) we failed to obtain Ura+ haploid.