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Localization of Spdd1p to the bands of polytene chromosomes. Macronuclear anlagen were fixed, squashed, incubated with the Pdd1p antiserum, and analyzed by immunofluorescence microscopy. (a–f, h) Anti-Pdd1p immunofluorescence stain (blue light); (g) phase-contrast image of h. Bar, 10 μm.
