Figure 4.

Sam68 nuclear bodies are dynamic structures. (A) HeLa cells were fixed, permeabilized, and immunostained with anti-Sam68 AD1 antibody followed by rhodamine-conjugated goat anti-rabbit secondary antibody, and the cell nuclei were stained with DAPI. Fluorescence of representative cells in interphase (a and f, lower cells; e and j, right cells), early prophase (a and f, large arrow), late prophase (b and g), metaphase (c and h), anaphase (d and i), and late telophase/early G1 phase (e and j, large arrows) are shown. Sam68 nuclear bodies are indicated by small arrows. (B) HeLa cells were mock-treated or treated with 5 μg/ml actinomycin D (b) or 20 μg/ml cycloheximide (c) for 3 h, then fixed, permeabilized, and immunostained with anti-Sam68 AD1 antibody.