Figure 7.

Characterization of GFP-Sam68ΔL1 nuclear bodies. (A) HeLa cells transfected with GFP-Sam68ΔL1 were fixed and analyzed with fluorescence and difference interference contrast (DIC) microscopy. (B) GFP-Sam68ΔL1 nuclear bodies are dynamic structures. HeLa cells were transfected with GFP-Sam68ΔL1 and then incubated at 37 or 32°C for 24 h; the last 3 h of incubation was performed with or without 5 μg/ml actinomycin D. The cells were fixed and visualized by fluorescence microscopy. (C) HeLa cells transfected with GFP-Sam68ΔL1 were fixed and observed 12, 24, 36, 48, or 60 h after transfection.