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Calcofluor sensitivity test. Yeast cells were resuspended at 106 cells/ml in sterile H2O. Serial dilutions of 1-, 5-, 25-, and 125-fold were made in sterile water, and 4 μl of each dilution were spotted on YPD plates containing the indicated concentration of CFW. The plates were incubated for 3 d at 24°C before photography. Strains LCY21 (SEY6210, WT), LCY24 (cwh41Δ), LCY22 (alg5Δ), and LCY23 (alg5Δcwh41Δ) represent a tetrad from strain LCY 20.
