Table 2.
Interaction between CWH41 and ALG5 genes
| Strain | Genotype | Killer toxin size (mm)a | Cell wall β-1, 6-glucan (μg/mg of cell wall dry weight)b |
|---|---|---|---|
| LCY21 | CWH41ALG5 | 13.0 ± 0.5 | 148 ± 17 |
| LCY24 | cwh41Δ | 7.0 ± 0.3 | 69 ± 12 |
| LCY22 | alg5Δ | 10.3 ± 0.5 | 129 ± 19 |
| LCY23 | cwh41Δalg5Δ | 10.5 ± 0.3 | 124 ± 23 |
Results represent the mean ± 1 SD of five independent determinations.
a
Sensitivity to K1 killer toxin was determined by seeded plate tests as described in MATERIALS AND METHODS.
b
Total cell walls were first isolated from stationary phase cells, and then the major cell wall polymers were fractionated and measured as described by Brown et al. (1994).