Figure 2.

In a meiotic time course, DNA content, nuclear area, nuclear fluorescence, and chromatin condensation were determined in wild type and top2 at 34°C. For detailed explanation see text and MATERIALS AND METHODS. (A) For every hour the percentage of cells that are in G₂ is shown. (B and C) The average relative nuclear area (B) and the average relative nuclear fluorescence (C) are shown for every hour. (D) The nuclear area divided by the DNA content gives a measure of chromatin condensation. (E) The timing of meiotic events in this particular time course is shown. wt, wild type; >1 nucleus, percentage of cells that performed the first meiotic division; Horse tail, percentage of cells that show the typical prophase nuclear morphology after DAPI staining.