Figure 4.

Transfection of α3 increases the cell surface expression of CD151. K562 cells transfected with α2, α3, α4, or α6 cDNA, or vector alone (K562-α5(Neo)) were stained with the negative control antibody, P3 (dotted lines), or with antibodies against β1 (TS2/16, first column), CD63 (6H1, second column) or CD151 (5C11, third column) before analysis by flow cytometry. Specific mean fluorescence intensity (MFI) values are indicated. Except for K562-α5(Neo) (in which α5β1 is the only β1 integrin), transfectants express similar levels of the respective α chains. The transfected α chains represent between 72 and 94% of total β1 integrin in these transfectants.