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. 1998 Oct;9(10):2751–2765. doi: 10.1091/mbc.9.10.2751

Figure 8.

Figure 8

Inhibition of PMN chemotaxis by antibody perturbation of α3β1 and CD151. PMN migration in response to fMLP was measured on fibronectin in the presence of β-glucan. mAbs A3-X8 (α3), 5C11 (CD151), C9-BB(CD9), 6H1 (CD63), 8A6 (CD98), and 4.6.19 (CD32) were incorporated into the agarose, as described in MATERIALS AND METHODS. Isotype-matched IgG and IgM served as controls. None of these antibodies triggered neutrophil homotypic aggregation. Migration distances, calculated from Microprojector-magnified images, are presented as mean ± SD from quadruplicate samples. Data are representative of three separate experiments. Background random migration values (no fMLP) ranged between 3–6 mm. mAbs against α3 and CD151 significantly inhibited PMN motility (p < 0.0001).