Figure 11.

The double mutant ste6–90,166 exhibits the same hyperunstable phenotype as the single mutant ste6–166. To compare the effect of the double mutation ste6–90,166 with the single mutations ste6–90 and ste6–166, we examined the metabolic stability of Ste6p encoded by these alleles by performing pulse-chase analysis, as described in Figure 4. Strains are SM2569 ([2μ STE6::HAe]), SM2919 ([2μ ste6–90::HAc]), SM3154 ([2μ ste6–90/166::HAe]), and SM3156 ([2 μ ste6–166::HAe]).