Table 2.
Plasmids used in this study
| Plasmid | Genotype | Reference |
|---|---|---|
| pRS316 | CEN URA3 | Sikorski and Hieter, 1989 |
| pSM192 | CEN URA3 STE6 | Berkower and Michaelis, 1991 |
| pSM217 | 2μ URA3 | Berkower and Michaelis, 1991 |
| pSM435 | 2μ LEU2 STE6 | Berkower and Michaelis, 1991 |
| pSM500 | 2μ LEU2 STE6::HAc | Paddon et al., 1996 |
| pSM683 | CEN URA3 STE6::HAe | Loayza and Michaelis, 1998 |
| pSM672 | 2μ URA3 STE6::HAc | Berkower, 1995 |
| pSM693 | 2μ URA3 STE6::HAe | Berkower et al., 1994 |
| pSM954 | YIp URA3 ste6-13::HAc | This study |
| pSM955 | YIp URA3 ste6-90::HAc | This study |
| pSM1080 | 2μ URA3 ste6-90,166::HAe | This study |
| pSM1081 | 2μ URA3 ste6-166::HAe | This study |
| pSM1082 | 2μ URA3 ste6-166::HAe | This study |
| pSM1083 | CEN URA3 ste6-166::HAe | This study |
| pSM1084 | CEN URA3 ste6-90::HAc | This study |
| pSM1085 | CEN URA3 STE6::HAc | Romano et al., 1998 |
| pSM1086 | 2μ URA3 ste6-13::HAc | This study |
| pSM1087 | 2μ URA3 ste6-90::HAc | This study |
| pSM1126 | CEN LEU2 ste6-166::HAe | This study |
| pSM1144 | CEN URA3 ste6-13::HA | This study |
| pSM1267 | 2μ URA3 containing HRD2 locus (from YEp24 library) | This study |
| pSM1347 | As pSM1267, with HRD2 gene inactivated at SacII site | This study |
| YEp105 | 2μ TRP1 ubi::myc | Hochstrasser et al., 1991 |