Figure 2.

Detection of SR1 and FGFR1 in neural retina. Two hundred micrograms of protein of neural retina extracts of adult rat were bound to ¹²⁵I-FGF2 heparin-Sepharose. After cross-linking with DSS, proteins were eluted, and immunoprecipitations was performed with antibodies raised against the specific amino sequence of SR1 (lane 2) and against the carboxyl terminus of the full-length FGFR1 (lane 3) as described in MATERIALS AND METHODS. Immunoprecipitated proteins were analyzed by SDS-PAGE on a 10% acrylamide gel and autoradiography. All lanes were run on the same gel, but irrelevant lanes were removed to improve clarity. The gel autoradiograms are representative of three independent experiments.