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High-copy suppressors of cdc15-2. Four of the cdc15-2 high copy suppressors (GAL-CDC15, GAL-SIC1, GAL-SPO12, and GAL-YAK1ΔN) were retransformed into a cdc15-2 strain (SLJ02), grown in YP/raffinose to midlog phase, serially diluted fivefold, and spotted onto YP/dextrose or YP/galactose-raffinose plates. Plates were incubated at 23 or 37°C for 2.5 d.
