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cut11ts alleles are synthetically lethal with the SPB mutant cut12–1ts. One example of limited growth is observed between cut11–5 and cut12–1, as shown. Top Left, the position of the parental strains, double mutant, and wild type on each of the plates. Top Right and Bottom, plates with strains grown on rich medium at 20, 25, and 36°C. The results with other allelic combinations are described in the text.
