Figure 5.

Effect of highly purified (A, B, and D) and high molecular weight HA (C and D) on MV3 cell migration within multicomponent matrices. Cells were embedded within matrices consisting of fibrillar collagen (col), or collagen supplemented with HA (1 mg/ml) (col+HA), or HA (1 mg/ml) and CS (20 mg/ml) (col+HA+CS). The spontaneous baseline migration in collagen as compared with multicomponent matrices was obtained from n = 3 independent experiments (A, 120 cells in total). C represents 40 cells (one representative experiment for n = 2). (B) Box plot analysis of cumulative velocities at the single-cell level, as derived from A. The lines within the boxes indicate the medians, and boxes represent range from 25th to 75th percentiles. Further scattering is indicated by whiskers (indicating values within 1.5 × box-length range), and extreme scattering values are indicated individually (●). (D) For dose–response to HA, the mean speed ± SD was assessed (40 cells, one representative experiment).