Figure 6.

CD44-independent baseline migration (A and B) and lack of CD44-mediated migratory restoration after blocking of β1 integrin function (C and D). Experiments were performed as described in Figure 5. Cells were preincubated with blocking mAbs (anti-CD44 mAb Hermes-1 and anti-β1 integrin mAb 4B4; 10 μg/ml) or PBS, embedded within the different matrices, and mAb was additionally added to supernatant and matrix. (B) Mean steady-state values of baseline velocity (▵) and percentage of migrating cells (□) in the absence (open symbols) or presence (closed symbols) of mAb Hermes-1. Data represent 120 cells (n = 3 experiments) in A and D and one representative experiment for n = 2 (B and C).