Figure 7.

Effect of adhesion-inducing anti-β1 integrin mAb 8A2 on MV3 cell migration (A and B), β1 integrin distribution (C, left), and interaction with collagen fibers (C, right). MV3 cells were embedded into collagen lattices. Three hours after the migratory onset mAb 8A2 was added to the supernatant (A, black arrow). After 24 h, samples were fixed, and 8A2-coated β1 integrins were stained with secondary LRSC-conjugated F(ab)′ fragments. Fluorescence (C, left) and reflection images (C, right) were detected by confocal microscopy. β1 integrin clustering and corresponding interactions with collagen fibers are indicated by white arrowheads. Bar, 20 μm.