Statistically significant differences of Akt signaling in plasma membrane microdomains. (A) Statistically significant differences between the responses from Figure 4, C–F. Each response is indicated as following: 1, PM(Lyn)-AktAR, stimulated with PDGF; 2, AktAR-PM(Kras), stimulated with PDGF; 3, PM(Lyn)-AktAR, stimulated with PDGF, preincubated with MβCD; 4, AktAR-PM(Kras), stimulated with PDGF, preincubated with MβCD; 5, PM(Lyn)-AktAR, stimulated with IGF-1; 6, AktAR-PM(Kras), stimulated with IGF-1; 7, PM(Lyn)-AktAR, stimulated with IGF-1, pre-incubated with MβCD; 8, AktAR-PM(Kras), stimulated with IGF-1, preincubated with MβCD. The asterisks show statistically significant difference with an unpaired student's t test (p < 0.05). (B) Statistically significant differences between the t1/2 value of the responses from Figure 4, C–F. The asterisks show statistically significant difference with an unpaired student's t test (p < 0.05).