Table 1.

Summary of in vitro GAP activity and in vivo genetic interactions in each Bfa1-DR2 mutant

Activity	Bfa1 mutants
	Wt Bfa1	G411E	D416A	M413I	W422A	Δbfa1
In vitro GAP activitya	100%					0%
The growth rate in Δlte1Δbfa1 cellsb	++	−	−	−	−	−
The growth rate in Δlte1Δste20Δbfa1cellsc	++	+	−	−	−	−
Premature binding of Mob1 to SPBs in cdc15–2Δbfa1 cellsd	++	++	++	+	+/−	−
The growth rate in cdc5–2Δbfa1cellse	++	++	++	++	+	−
Spindle damage checkpoint activityf	++	+	−	−	−	−
DNA damage checkpoint activityg	++	++	+	+	−	−
Spindle position checkpoint activityh	++	++	−	+	++	−

++ indicates nearly activity similar to wild-type Bfa1, and the order of Bfa1 activity is denoted as follows: ++ > + > +/− > −.

^a Figure 3E;

^b Figure 2, A and B;

^c Supplementary Figure S5;

^d Supplementary Figure S6;

^e Figure 2, C and D;

^f Figure 4, B and C;

^g Figure 4E; and

^h Figure 5.