Figure 9.
Synthetic genetic interactions between certain cdc42ts alleles and swf1Δ required the Swf1p DHHC motif. Equivalent dilutions of swf1Δ cdc42-118, swf1Δ cdc42-129, and swf1Δ cdc42-101 strains were plated and grown at 25, 30, and 37°C for 3 d on SC-Ura. These strains contained a CEN plasmid vector that was either empty or filled with wild-type SWF1 (DHHC) or swf1 in which the DHHC motif was mutated (DHHA). The strains shown (from top to bottom) are KKY1137, 1138, 1139, 1140, 1134, 1135, and 1136. Note that no swf1Δ cdc42-129 cells containing vector or plasmid-borne swf1-DHHA were plated because neither the vector nor swf1-DHHA suppressed the synthetic lethal interaction between swf1Δ and cdc42-129.