Table 1.
S. cerevisiae strains used in this study
| Strain | Relevant genotype | Source |
|---|---|---|
| DDY1102 (alias KKY49) | MATa/MATα SWF1/SWF1 ura3-52/ura3-52 his3Δ200/his3Δ200 leu2-3,112/leu2-3,112 ADE2/ade2-1 lys2-801/LYS2 | Kozminski et al. (2000) |
| KKY281 | MATα CDC42:natMX can1Δ::MFA1pr-HIS3-MFα1pr-LEU2 his3Δ1 leu2Δ0 ura3Δ0 lys2Δ0 | Kozminski et al. (2003) |
| KKY282 | MATα cdc42-101:natMX can1Δ::MFA1pr-HIS3-MFα1pr-LEU2 his3Δ1 leu2Δ0 ura3Δ0 lys2Δ0 | This studya |
| KKY283 | MATα cdc42-118:natMX can1Δ::MFA1pr-HIS3-MFα1pr-LEU2 his3Δ1 leu2Δ0 ura3Δ0 lys2Δ0 | Kozminski et al. (2003) |
| KKY293 | MATα cdc42-129:natMX can1Δ::MFA1pr-HIS3-MFα1pr-LEU2 his3Δ1 leu2Δ0 ura3Δ0 lys2Δ0 | This studya |
| KKY1060 | MATa/MATα swf1Δ::kanMX4/swf1Δ::kanMX4 ura3-52/ura3-52 his3Δ200/his3Δ200 leu2-3,112/leu2-3,112 ADE2/ade2-1 lys2-801/lys2-801 | KKY1063 × KKY1065 |
| KKY1062 | MATa/MATα SWF1/swf1Δ::kanMX4 ura3-52/ura3-52 his3Δ200/his3Δ200 leu2-3,112/leu2-3,112 ADE2/ade2-1 lys2-801/lys2-801 | KKY1064 × KKY1065 |
| KKY1063 | MATα swf1Δ::kanMX4 ura3-52 his3Δ200 leu2-3,112 lys2-801 | Meiotic product of KKY1074 |
| KKY1064 | MATα ura3-52 his3Δ200 leu2-3,112 lys2-801 | Meiotic product of KKY1074 |
| KKY1065 | MATaswf1Δ::kanMX4 ura3-52 his3Δ200 leu2-3,112 ade2-1 lys2-801 | Meiotic product of KKY1074 |
| KKY1067 | MATaswf1Δ::kanMX4 CDC42:natMX his3Δ1 leu2Δ0 ura3Δ0 lys2Δ0 can1Δ::MFA1pr-HIS3-MFα1pr-LEU2 | Meiotic product of KKY1145 |
| KKY1068 | MATaswf1Δ::kanMX4 cdc42-118:natMX his3Δ1 leu2Δ0 ura3Δ0 lys2Δ0 can1Δ::MFA1pr-HIS3-MFα1pr-LEU2 | Meiotic product of KKY1144 |
| KKY1069 | MATa/MATα swf1Δ::kanMX4/SWF1 CDC42/cdc42-129:natMX his3Δ1/his3Δ1 leu2Δ0/leu2Δ0 ura3Δ0/ura3Δ0 lys2Δ0/LYS2 CAN1/can1Δ::MFA1pr-HIS3-MFα1pr-LEU2 met15Δ0/MET15 | KKY293 × 1-7-11 |
| KKY1070 | MATaswf1Δ::kanMX4 cdc42-101:natMX his3Δ1 leu2Δ0 ura3Δ0 lys2Δ0 can1Δ::MFA1pr-HIS3-MFα1pr-LEU2 | Meiotic product of KKY1143 |
| KKY1074 | MATa/MATα swf1Δ::kanMX4/SWF1 his3Δ200/his3Δ200 leu2-3,112/leu2-3,112 ura3-52/ura3-52 ADE2/ade2-1 lys2-801/LYS2 | This studya |
| KKY1084 | KKY1064 [pKK1586, YCplac33(SWF1)] | This studyb |
| KKY1085 | KKY1064 [pKK1873, YCplac33(swf1-DHHA)] | This studyb |
| KKY1086 | KKY1063 [pKK1586,YCplac33(SWF1)] | This studyb |
| KKY1087 | KKY1063 [pKK1873, YCplac33(swf1-DHHA)] | This studyb |
| KKY1088 | MATapfy1-4:LEU2 ura3-52 his3Δ200 leu2-3,112 lys2-801 | This studyc |
| KKY1090 | MATα swf1Δ::kanMX4 ura3-52 his3Δ200 leu2-3,112 lys2-801 | This studyd |
| KKY1104 | MATa/MATα SWF1-GFP:kanMX4/SWF1 his3Δ200/his3Δ200 ura3-52/ura3-52 leu2-3,112/leu2-3,112 ADE2/ade2-1 lys2-801/LYS2 | This studya |
| KKY1120 | MATa/MATα his3Δ1/his3Δ1 leu2Δ0/leu2Δ0 LYS2/lys2Δ0 met15Δ0/MET15 | BY4741 × BY4742 |
| KKY1134 | MATaswf1Δ::kanMX4 cdc42-101:natMX his3Δ1 leu2Δ0 ura3Δ0 lys2Δ0 can1Δ::MFA1pr-HIS3-MFα1pr-LEU2 [YCplac33] | This studye |
| KKY1135 | MATaswf1Δ::kanMX4 cdc42-101:natMX his3Δ1 leu2Δ0 ura3Δ0 lys2Δ0 can1Δ::MFA1pr-HIS3-MFα1pr-LEU2 [pKK1586,YCplac33(SWF1)] | This studye |
| KKY1136 | MATaswf1Δ::kanMX4 cdc42-101:natMX his3Δ1 leu2Δ0 ura3Δ0 lys2Δ0 can1Δ::MFA1pr-HIS3-MFα1pr-LEU2 [pKK1873, YCplac33(swf1-DHHA)] | This studye |
| KKY1137 | KKY1068 [YCplac33] | This studyb |
| KKY1138 | KKY1068 [pKK1586,YCplac33(SWF1)] | This studyb |
| KKY1139 | KKY1068 [pKK1873, YCplac33(swf1-DHHA)] | This studyb |
| KKY1140 | MATaswf1Δ::kanMX4 cdc42-129:natMX his3Δ1 leu2Δ0 ura3Δ0 lys2Δ0 can1Δ::MFA1pr-HIS3-MFα1pr-LEU [pKK1586,YCplac33(SWF1)] | This studyf |
| KKY1141 | KKY1069 [YCplac33] | This studyb |
| KKY1142 | KKY1069 [pKK1873, YCplac33(swf1-DHHA)] | This studyb |
| KKY1143 | MATa/MATα swf1Δ::kanMX4/SWF1 CDC42/cdc42-101:natMX his3Δ1/his3Δ1 leu2Δ0/leu2Δ0 ura3Δ0/ura3Δ0 lys2Δ0/lys2Δ0 CAN1/can1Δ::MFA1pr-HIS3-MFα1pr-LEU2 met15Δ0/MET15 | KKY282 × 1-7-11 |
| KKY1144 | MATa/MATα swf1Δ::kanMX4/SWF1 CDC42/cdc42-118:natMX his3 Δ1/his3Δ1 leu2Δ0/leu2Δ0 ura3Δ0/ura3Δ0 lys2Δ0/lys2Δ0 CAN1/can1Δ::MFA1pr-HIS3-MFα1pr-LEU2 met15Δ0/MET15 | KKY283 × 1-7-11 |
| KKY1145 | MATa/MATα swf1Δ::kanMX4/SWF1 CDC42/CDC42:natMX his3Δ1/his3Δ1 leu2Δ0/leu2Δ0 ura3Δ0/ura3Δ0 lys2Δ0/lys2Δ0 CAN1/can1Δ::MFA1pr-HIS3-MFα1pr-LEU2 met15Δ0/MET15 | KKY281 × 1-7-11 |
| KKY1146 | MATaswf1Δ::kanMX4 ura3-52 his3Δ200 leu2-3,112 lys2-801 | Meiotic product of KKY1074 |
| KKY1147 | MATaura3-52 his3Δ200 leu2-3,112 lys2-801 | Meiotic product of KKY1074 |
| DDY663 | MATα ura3-52 leu2-3,112 lys2-801 | D. Drubing |
| DDY664 | MATaura3-52 leu2-3,112 lys2-801 | D. Drubing |
| DDY1300 | MATaCDC42:LEU2 ura3-52 leu2-3,112 his3Δ200 lys2-801 | Kozminski et al. (2000) |
| DDY1304 | MATacdc42-101:LEU2 ura3-52 leu2-3,112 his3Δ200 lys2-801 | Kozminski et al. (2000) |
| DDY1326 | MATacdc42-118:LEU2 ura3-52 leu2-3,112 his3Δ200 lys2-801 | Kozminski et al. (2000) |
| DDY1336 | MATacdc42-123:LEU2 ura3-52 leu2-3,112 his3Δ200 lys2-801 | Kozminski et al., (2000) |
| DDY1344 | MATacdc42-129:LEU2 ura3-52 his3Δ200 leu2-3,112 lys2-801 | Kozminski et al. (2000) |
| DDY2008 | MATapfy1-4:LEU2 ura3-52 his3Δ200 leu2-3,112 lys2-801 | Wolven et al. (2000) |
| 1-7-11 | MATaswf1Δ::kanMX4 ura3Δ0 his3Δ1 leu2Δ0 met15Δ0 | Winzeler et al. (1999) |
| BY4741 | MATahis3Δ1 leu2Δ0 ura3Δ0 met15Δ0 | Brachmann et al. (1998) |
| BY4742 | MATα his3Δ1 leu2Δ0 ura3Δ0 lys2Δ0 | Brachmann et al. (1998) |
| NY17 | MATasec6-4 ura3-52 | Novick et al. (1980) |
| Y3611 | MATa/MATα can1Δ::MFA1pr-HIS3-MFα1pr-LEU2/CAN1 his3Δ1/his3Δ1 leu2Δ0/leu2Δ0 ura3Δ0/ura3Δ0 met15Δ0/MET15 lys2Δ0/LYS2 | Kozminski et al. (2003) |
a See Materials and Methods.
b By transformation with indicated plasmid.
c Progeny of backcross of DDY2008 with DDY663.
d Progeny of backcross of KKY1063 with DDY663.
e Meiotic product of KKY1143 transformed with indicated plasmid.
f Meiotic product of KKY1069 transformed with indicated plasmid.
g University of California at Berkeley.