Figure 7.

The phosphorylation domain of HDAC5 is required for its interaction with YY1, and YY1 blunts HDAC5 phosphorylation. (A and B) Overexpression of YY1 blunts HDAC5 phosphorylation in response to PE. (A) NRVMs were infected with CMV or YY1-GFP. Cells were treated with PE for 1 h and total protein was extracted. Phosphorylated Ser 259 HDAC5 and total HDAC5 were detected by Western blot, and calnexin antibody was used as a loading control. (B) Quantification of Western in A. (C and D) COS cells were transfected with YY1-GAL and wild-type HDAC5-MYC (C) or YY1-GAL and HDAC5 del 260-615-FLAG (D), and total protein was extracted. Immunoprecipitation was done with a GFP antibody (control) or a GAL4 antibody, and HDAC5 was detected by Western blot using either MYC or FLAG antibodies. N.S., nonspecific.