Figure 2.

Properties of microtubule arrays in tendon cells. Horizontal views of muscles (white dots) and tendon cells (asterisks) taken in intermediate (i) or basal (b) focal planes, according to Figure 1B. Usual networks of microtubules at the surface of muscles and tendon cells (A¹) are severely reduced or abolished upon nocodazole treatment (B¹); curved arrows point at nerves reaching into the image. In control animals, cytoskeletal arrays are labeled with tubulin::GFP (magenta), whereas anti-tubulin staining mostly fails to penetrate deep into tendon cells (A²); in nocodazole-treated animals the cytoskeletal belts are perfectly labeled by both anti-tubulin and tubulin::GFP (B²). Cytoskeletal belts can also be labeled with other anti-tubulin antibodies (E; Glu, anti-glu-tubulin; Tyr, anti-tyrosinated tubulin) or tubulin-associated proteins: Short stop::GFP (ShotGFP; F), antibody staining against C-terminal Short stop (ShotC; G) and EB1::GFP (EB1GFP; H). Bar is 10 μm (A and B) and 17 μm (E–H).