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. 2008 Oct;19(10):4287–4297. doi: 10.1091/mbc.E08-02-0182

Figure 6.

Figure 6.

Further properties of tendon cells. Direct muscle attachments (white dots, muscles; asterisks, and tendon cells), labeled as indicated bottom left: Sqh, Spaghetti squash; Zip, Zipper; Ena, Enabled; EnaGFP, Enabled::GFP; act, actin::GFP; Phal, Phalloidin; anterior is left. (A–B′) Sqh::GFP localizes to the cytoskeletal belt (between arrow heads in A1) and the apical fields of cytoskeletal arrays (A2); Zipper colocalizes with Sqh at the cytoskeletal belt (B). (C) Enabled is enriched in late larval tendon cells (asterisk), but it does not concentrate at cytoskeletal arrays. (D) Enabled::GFP is also absent from cytoskeletal arrays. (E–H) In late embryonic tendon cells, Ena accumulates at the tendon cell periphery of wild type (white arrows in E) but not enaGC1/23 mutant embryos (F); absence of Enabled has no obvious effect on the F-actin arrays (G vs. H). Bar, 10 μm (A, C, and D) 6 μm (B), and 7 μm (E–H).