Figure 7.

Persistence of F-actin arrays in the absence of Rho1 or integrin function. Live images of embryos (wt, wild type; mys, myospheroid mutant; Rho-IR/RhoN19, tendon cell-specific expression of Rho1 iRNA/RhoN19) with tendon-cell specific expression of GFP-tagged proteins (act, actin; tub, tubulin); anterior up in A–C and left in D–F′. Cytoskeletal arrays are hardly affected by loss of Rho1 function (C and D) and are arranged into rings in myospheroid mutant embryos (arrowheads in D′, E′, and F′). Most pictures are taken of indirect muscle attachments in the dorsal muscle field, where the cytoskeletal arrays form one anterior and one posterior cytoskeletal belt (arrows in D). Bar, 14 μm.