Figure 1.

Domain organization of the Mif2p and CENP-C polypeptide chains. Top, the Mif2p polypeptide chain. Yellow, green, red, and blue blocks indicate regions of distinct function (assigned, in part, through results described herein). The two regions recognizably conserved in human CENP-C (middle) are a short “CENP-C signature sequence” (light green) and a C-terminal domain (blue) shown here to be a dimerizing element. The pink box in the human CENP-C diagram represents the presumptive DNA-binding domain. Various signature-motif sequences are shown in the bottom panel.