. 2008 Oct;19(10):4480–4491. doi: 10.1091/mbc.E08-03-0297

Table 2.

Recruitment to centromeres of various kinetochore components, determined by ChIP and/or fluorescence microscopy in various kinetochore-mutant backgrounds

Mutant background	Mutated protein (complex)	Analyzed protein (complex)	Temp		Positive control (Cep3p)
Mutant background	Mutated protein (complex)	Analyzed protein (complex)	24°C	37°C	24°C	37°C
cse4–1	Cse4-Scm3 nucleosome	Mif2p (Mif2p dimer)	+^a	−^a	+^a	+^a
		Okp1p-GFP (COMA)	+^a	−^a	+^a	+^a
		Mtw1p-GFP (MIND)	+^a	−^a	+^a	+^a
		Ndc80p-GFP (Ndc80 complex)	+	+	+	+
		Spc105p-GFP (Spc105 complex)	+	+	+	+
mif2–3	Mif2p dimer	Cse4p-GFP (Cse4p-Scm3p nucleosome)	+	+	n.d.^b	n.d.
		Spc105p-GFP	+	+	+	+
mif2^td	Mif2p dimer	Mtw1p-GFP	+^a	−^a	n.d.	n.d.
		Ndc80p-GFP	+	+	n.d.	n.d.
		Ctf19p-GFP (COMA)	+^a	−^a	n.d.	n.d.
ndc80–1	Ndc80 complex	Mif2p-TAP	+^c	+^c	n.d.	n.d.
		Spc105p-GFP	+	+	+	+
spc105–15	Spc105 complex	Mif2p	+	+	+	+
		Okp1p-GFP	+	+	+	+
		Mtw1p-GFP	+	+	+	+
		Ndc80p-GFP	+	+	+	+

+, protein localizes to CENIV (ChIP) and/or kinetochores (fluorescence microscopy). Recruitment of all proteins was scored by ChIP, except for Cse4p-GFP in mif2–3 and Mtw1p-GFP, Ndc80p-GFP, and Cftf19p-GFP in the Mif2p degron strain mif2^td, for which recruitment was analyzed using wide-field Deltavision fluorescence microscopy (De Wulf et al., 2003). −, protein does not localize to CENIV (ChIP).

^a Confirms a previous result reported by Westermann et al. (2003).

^b n.d., not determined.

^c See Westermann et al. (2003).