Figure 1.

Efg1 interacts with NuA4 in yeast and hyphae. (A) Yeast two-hybrid assays. EGY48 (p8op-lacZ) was cotransformed with plasmids as indicated. (B) Coimmunoprecipitation of Efg1 interacts with Eaf7, Epl1, and Yng2 in yeast and hyphae. C. albicans strains CLY5 (Efg1-TAP Eaf7-myc) and CLY6 (Eaf7-myc), CLY7 (Efg1-TAP Epl1-myc) and CLY8 (Epl1-myc), or CLY9 (Efg1-TAP Yng2-myc) and CLY10 (Yng2-myc) were grown at 25°C in YPD for yeast growth and at 37°C in YPD + 10% serum for hyphal growth. Protein lysates were subjected to immunoprecipitation with IgG beads (GE Healthcare), and the precipitated proteins were separated by 7.5% SDS-PAGE and probed with peroxidase-conjugated anti-c-myc (Sigma-Aldrich). As input control, cell lysates were analyzed by Western blotting with the peroxidase-conjugated anti-c-myc.