Figure 5.

Nucleosomal H4 acetylation levels at the promoters of hypha-specific genes increase rapidly during initial hyphal induction in wild-type but not in efg1/efg1 strain. Cells of SC5314 (WT) and HLC52 (efg1) were transferred into 10% serum in YPD at 37°C for hyphal induction, and cells were collected at 0 min, 15 min, 30 min, 1 h, 2 h, and 3 h for ChIP with anti-histone H4 and anti-acetylated H4 antibodies. The amounts of HWP1, ALS3, and ECE1 upstream DNA in the ChIP reactions were determined by quantitative PCR with primers at −1381 to −1101 of HWP1, −424 to −290 of ALS3, and −1350 to −1103 of ECE1. Left, levels of H4 (relative H4 occupancy) at these promoters, which are normalized to the respective control DNA at the ADE2 promoter. The 0-min values in the wild-type strain are set to be 1.00. Acetylation levels normalized with respect to H4 levels are presented (right; H4 acetylation/H4 occupancy). They are calculated by dividing the values for acetylated H4 with H4 occupancy values.