Figure 8.

Suppression of Kaiso protein expression in p120i cells restores H. pylori-mediated increases in mmp-7 mRNA levels. Control or p120i cells transiently transfected with scrambled siRNA, and p120i cells transiently transfected with Kaiso-specific siRNA were cocultured with H. pylori strain 7.13 (MOI = 100) or medium alone. At defined time points, total RNA was extracted, subjected to reverse transcription, and analyzed in triplicate by real-time PCR. (A) Data are represented as an average percentage of kaiso expression in uninfected p120i cells at each time point for experiments performed on at least three occasions. (B) Data are represented as the percent maximum induction of mmp-7 expression in infected versus uninfected cells for experiments performed on at least three occasions. Error bars, SEM. *p < 0.05 and **p < 0.01 versus p120i cells.