Figure 1.

Inactivation of MNL1 causes a defect in weak acid stress responses. C. albicans strains were grown on solid SC-pH 3.0 medium containing weak acids applied on filter discs. All strains were transformed with the URA3-containing plasmid, CIp10. (A) Growth of C. albicans strains on medium containing varying concentrations of acetic acid (mM): wild type, CAI8; mnl1, MSC4; msn4, MSC8; mnl1 msn4, MSC12 (Table 1). (B) Effects of other weak acids on wild-type (CAI8) and mnl1 strains (MSC4): formic (7.5 M), and acetic; butyric; sorbic and benzoic acids (0.5–7.5 M).