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. 2008 Oct;19(10):4383–4392. doi: 10.1091/mbc.E08-02-0219

Figure 1.

Figure 1.

Ago2, 3, and 4 transcripts are expressed in oocytes and at early stages of development, with Ago2 and 3 transcripts in all preimplantation stages tested. (A) PCR analysis of Ago1-4 gene family on preimplantation mouse first-strand cDNA from different stages. As a positive control for the PCR amplification, a band was successfully amplified for all Argonautes in ES cells. Actin and tubulin were used as control genes. (B) Localization of Ago2-GFP fusion protein in mRNA decay centers. Confocal laser microscopy images of two-cell embryos microinjected at zygote stage with tagged mRNA for Ago2-GFP and Dcp1a-DsRed. Top panel, vesicle-like localization of Ago2-GFP and Dcp1a-DsRed fusion proteins in a two-cell embryo (96 μM × 96 μM). Bottom panel, a higher magnification of one blastomeres in the two-cell embryo expressing Ago2-GFP and DsRed-Dcp1a (46 μM × 46 μM). Green, red, and orange arrowheads point to examples of vesicles showing localization of Ago2-GFP, DsRed-Dcpla, and Ago2-GFP+DsRed-Dcpla, respectively.