Figure 1.

Two-dimensional gel analysis of flagellar proteins. Proteins from long and short flagella samples were fluorescently labeled and then analyzed on the same 2D gel. (A) Cy3 image of a 2D gel showing the proteins from full-length flagella. (B) Cy5 image of the same 2D gel showing the proteins from short, regenerating flagella. The similar shape and intensity of the tubulin (T) region of each image indicates the same amount of total flagellar protein was loaded for each sample. Numbers 1–4 indicate proteins referred to in Results. The small circles with plus signs in the center (two per gel image) were used for image alignment.