Figure 6.

Protein methylation varies with the state of the flagella. Flagella were isolated from full-length, regenerating, and resorbing flagella, fractionated into freeze-thaw (FT), membrane (M), and axoneme (Axo) fractions, and analyzed by immunoblotting using antibodies to asymmetrically dimethylated arginine residues (top panel). The migration of standards is shown to the left (×10⁻³), and the masses (kDa) of proteins A–D, estimated from the migrations of the standards, are shown to the right. The blot was then stripped and probed with antibodies to α-tubulin to serve as a loading control (bottom panel).