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. 2008 Oct;19(10):4492–4505. doi: 10.1091/mbc.E08-04-0363

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© 2008 by The American Society for Cell Biology

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Figure 2. — Efficient PMN requires the core autophagic machinery, consisting of (A) the two ubiquitin-like conjugation systems, coupling Atg12 to Atg5 and Atg8 to phosphatidylethanolamine; (B) the Atg9 cycling system except for Atg23 and Atg27; and (C) the phosphatidylinositol 3-kinase complex I. PMN further requires Atg1, Atg13, and most of the accessory Atg proteins (D). Atg17, Atg29, and Atg31 are required only for unselective macroautophagy, but not the selective Cvt pathway. Atg11 and the sorting nexins Atg20/Snx42 and Atg24/Snx4 are specifically required for the Cvt pathway. Please note that not all interactions in the drawing must take place simultaneously. (E) Atg21 and the proaminopeptidase I-receptor Atg19 are Cvt pathway–specific components. The function of the sterol glucosyltransferase Atg26 is restricted to pexophagy in P. pastoris. Further details are given in the text. Cells chromosomally deleted for the indicated genes and expressing GFP-Osh1 were starved in SD(-N) medium, and the increase in free GFP levels was monitored in Western blots as described in Figure 1. Cytosolic 3-phosphoglycerate kinase (PGK) was detected as a loading control. As noted cells from two different strain backgrounds (BY4741 or WCG) were analyzed.

Figure 2. — Efficient PMN requires the core autophagic machinery, consisting of (A) the two ubiquitin-like conjugation systems, coupling Atg12 to Atg5 and Atg8 to phosphatidylethanolamine; (B) the Atg9 cycling system except for Atg23 and Atg27; and (C) the phosphatidylinositol 3-kinase complex I. PMN further requires Atg1, Atg13, and most of the accessory Atg proteins (D). Atg17, Atg29, and Atg31 are required only for unselective macroautophagy, but not the selective Cvt pathway. Atg11 and the sorting nexins Atg20/Snx42 and Atg24/Snx4 are specifically required for the Cvt pathway. Please note that not all interactions in the drawing must take place simultaneously. (E) Atg21 and the proaminopeptidase I-receptor Atg19 are Cvt pathway–specific components. The function of the sterol glucosyltransferase Atg26 is restricted to pexophagy in P. pastoris. Further details are given in the text. Cells chromosomally deleted for the indicated genes and expressing GFP-Osh1 were starved in SD(-N) medium, and the increase in free GFP levels was monitored in Western blots as described in Figure 1. Cytosolic 3-phosphoglycerate kinase (PGK) was detected as a loading control. As noted cells from two different strain backgrounds (BY4741 or WCG) were analyzed.