Figure 8.

Formation of nucleus-vacuole (NV) junctions does not depend on Atg18, a core autophagy protein. In fluorescence microscopy NV junctions can be detected by the accumulation of GFP-Osh1 in regions between the nucleus and the vacuolar membrane (white arrowheads). The frequency of cells with NV junctions was counted and expressed as percentage of the total number of cells. In atg18Δ cells the frequency of NV junctions was WT like. Bar, 10 μm. From bottom to top, Nomarski optics, merge, nuclear staining with Hoechst 33342, and GFP fluorescence.