Figure 1.

Analysis of the rid mutant phenotype. Fivefold serial dilutions of initiation or elongation defective mutants (as indicated) in the absence or presence of Δcds1 and Δchk1 were plated on YEA plates and incubated for 3–5 d at the indicated critical temperature. Wild-type, Δcds1, and Δchk1 cells were included as controls. After shift to the critical temperature in the absence of Chk1, the viability of rid mutants was dramatically decreased compared with rid mutants lacking Cds1 or control strains. In contrast, the viability of elongation mutants is dependent on Cds1.