Skip to main content
. 2008 Oct;19(10):4534–4544. doi: 10.1091/mbc.E08-04-0341

Figure 1.

Figure 1.

AtMAP65-5 bundles MTs in vitro. (a–c) Observations of rhodamine-labeled taxol-stabilized MTs (1 μM) incubated in the absence (a) or in the presence of AtMAP65-5 (b) and AtMAP65-1 (c) at 0.5 μM. AtMAP65-1 and AtMAP65-5 induce MT bundling. Scale bar, 10 μm. (d–h) Negatively stained electron microscopy of MTs alone (d), with AtMAP65-5 (e and g) and with AtMAP65-1 (f and h). Low-magnification view of MTs and bundled MTs (d, e, and f, respectively). Scale bar, 75 nm. (g and h) High-magnification view of aligned MTs shows AtMAP65-5 (g) and AtMAP65-1 (h) forming extensive inter-MT bridges oriented with an angle of ∼50° relative to the axis of the MTs. Scale bar, 50 nm.