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. Author manuscript; available in PMC: 2008 Sep 29.
Published in final edited form as: Development. 2007 Dec 12;135(2):377–385. doi: 10.1242/dev.015081

Figure 2. Shh expression reveals the presence of ectopic pre-ameloblasts in Spry4-/-;Spry2+/-, in Spry4-/-, and in Fst-/- embryonic incisors.

Figure 2

(A-I) Gene expression analyzed by RNA in situ hybridization using the indicated probes on paraffin sections of wild-type and mutant embryonic incisors. The stage at which the embryos were collected is indicated. In this and subsequent figures, a dotted line outlines the incisor epithelium. In panel D, the Shh expression domain appears to extend slightly into the lingual epithelium, but as this was not reproducibly observed it is likely an artifact of the plane of section. The red arrowheads in panels E and H point to a domain in which Shh is ectopically expressed on the lingual side of Spry4-/-;Spry2+/- and Spry4-/- incisors. A similar domain is detected in Fst null incisors (panel I). The open arrowheads in panel I point to an additional anterior domain in which Shh is ectopically expressed on the lingual side of Fst null incisors. Abbreviations as in the legend to Fig. 1. Scale bars = 100 μm.