Table 1.
cGMP-bound wild type | R307E | R307W | |
---|---|---|---|
Space Group | P21 | P21 | P21212 |
Resolution (Å) | 50.0-2.35 | 50.0-2.20* | 41.09-2.90 |
Rwork (%) | 20.9 | 20.2* | 26.0 |
Rfree (%) | 25.6 | 22.0* | 30.6 |
Rmsd bond length (Å) | 0.009 | 0.01 | 0.006 |
Rmsd bond angle (°) | 1.3 | 1.2 | 0.95 |
Completeness (%) | 90.0 (94.0) | 89.9(96.0) * | 99.3 (97.7) |
Rsym (%) | 8.0 (7.9) | 7.3 (28.4) | 6.6 (29.6) |
I/Sig I | 10.0 (7.0) | 8.0 (2.3) | 24.0 (5.3) |
Redundancy | 3.3 (3.2) | 4.1(2.9) | 4.8 (4.6) |
During refinement data included reflections to 2.0 Å. Due to outer shell incompleteness, refinement statistics and completeness are reported to 2.2 Å only.