Figure 5.

The speed of polymerization-coupled movement away from a chromosome is similar to the rate of growth at free microtubule plus ends. Bars are SEs, and the value in parentheses adjacent to each data point indicates the number of events used in calculating that point. Filled squares, Polymerization-coupled movement of brightly labeled microtubule segments away from the chromosome to which they are tethered; open circles, growth at the plus ends of axoneme-seeded microtubules; open triangle, growth at the plus ends of axoneme-seeded microtubules in the presence of 10% rhodamine-labeled tubulin (n = 4). Note that the open triangle was shifted to the right for clarity of display; the total concentration of labeled and unlabeled tubulin was 0.75 mg/ml.