TABLE 2.
Effect of amino acids substitution on kinetic properties of FAOX-II ND, not detected (detection limit is 0.1 nmol/min/mg). Fructosyl-glysine is used as a substrate.
| Km | Vmax | Structural role | |
|---|---|---|---|
| mm | mmol/min/mg | ||
| FAOX-II (wild type) | 0.24 | 3.30 | |
| E280L | ND | Fructosyl binding | |
| R411L | ND | ||
| Y60F | 0.86 ± 0.09 | 4.25 ± 0.23 | Carboxylate binding |
| R112E | 2.45 ± 0.18 | 3.97 ± 0.21 | |
| K368M | 1.25 ± 0.11 | 4.36 ± 0.16 | |
| E71S | 0.20 ± 0.07 | 2.59 ± 0.09 | Salt bridge between flexible regions |
| E71R | 0.21 ± 0.05 | 4.41 ± 0.31 | |
| R114E | 2.50 ± 0.14 | 2.94 ± 0.28 | |
| R114S | 0.18 ± 0.05 | 2.05 ± 0.13 | |
| E71R/R114E | 2.06 ± 0.18 | 3.92 ± 0.37 |