Effects of heNOS phosphorylation on Ca2+,
CaM activation, or Cav-P inhibition of
generation
generation rates from control and Akt or PKCα-phosphorylated heNOS
(BH4-free) were measured by EPR spin-trapping as described in
Fig. 2 with EC50
measured as in Figs. 4 and
6. The effect of
phosphorylation of heNOS on the interaction with Cav-P was determined with
Cav-P inhibition expressed as the percent inhibition produced by the addition
of Cav-P (400 μm) to a reaction containing 500 μm
Ca2+, 600 nm CaM.