TABLE 1.

Effects of heNOS phosphorylation on Ca²⁺, CaM activation, or Cav-P inhibition of generation generation rates from control and Akt or PKCα-phosphorylated heNOS (BH₄-free) were measured by EPR spin-trapping as described in Fig. 2 with EC₅₀ measured as in Figs. 4 and 6. The effect of phosphorylation of heNOS on the interaction with Cav-P was determined with Cav-P inhibition expressed as the percent inhibition produced by the addition of Cav-P (400 μm) to a reaction containing 500 μm Ca²⁺, 600 nm CaM.

	EC50 of [calcium]	EC50 of [CaM]	Cav-P inhibition of superoxide generation
	μm	nm	%
Control	22.1	208	35.7 ± 3.9
Akt	4.5	149	50.6 ± 2.6a
PKCα	23.2	496	67.8 ± 1.6b

^ap < 0.02 versus control

^bp < 0.01 versus control. Data are expressed as the mean ± S.E., n = 3