TABLE 1.

Lifetimes for donor-only (D) and donor:acceptor (DA)-tagged GluR6 ligand binding domain constructs and distances determined based on these lifetimes

Protein	Ligated state	τD	τDA	Distance
		μs	μs	Å
S398C-S666C	Apo	1699 ± 27	192 ± 2	42.5 ± 0.7
	Glutamate	1745 ± 32	130 ± 3	39.4 ± 0.9
	Kainate	1753 ± 34	149 ± 4	40.3 ± 1
	Domoate	1712 ± 30	144 ± 3	40.3 ± 1
	Methyl glutamate	1675 ± 27	129 ± 3	39.6 ± 0.9
S398C-S689C	Apo	1745 ± 24	185 ± 2	42.0 ± 0.6
	Glutamate	1774 ± 12	84 ± 2	36.3 ± 0.9
	Kainate	1718 ± 23	97 ± 2	37.5 ± 0.8
	Domoate	1684 ± 24	96 ± 2	37.5 ± 0.8
	Methyl glutamate	1721 ± 22	75 ± 2	35.8 ± 1
S398C-S666C-T661E	Apo	1786 ± 24	212 ± 3	42.9 ± 0.6
	Glutamate	1733 ± 32	135 ± 3	39.7 ± 0.9
	Kainate	1777 ± 25	174 ± 3	41.4 ± 0.6
S398C-S689C-T661E	Apo	1711 ± 30	176 ± 2	41.8 ± 0.7
	Glutamate	1698 ± 34	66 ± 1	35.1 ± 0.7
	Kainate	1690 ± 31	98 ± 2	37.7 ± 0.8