FIGURE 7.

Klf3 can repress activation mediated by Klf1 at Klf8 promoter 1a (A) and Klf8 promoter 1c (B) constructs. SL-2 cells were transfected with 1 μg of pGL4.10[luc2] containing regions of Klf8 promoter 1a or promoter 1c. Constructs cloned into the luciferase vector contained no CACCC sites to all CACCC sites. Empty pGL4.10[luc2] was used as a control. 1 μg of pPac.Eklf was used to activate promoter constructs. Increasing concentrations of pPac.Bklf (0.01, 0.1, or 1 μg) were used to repress promoter constructs. 1 μg of Renilla vector pGL4.74[HRluc/TK] was used as a transfection control. The average of four to six replicates/column is shown. The error bars show S.E. Dark closed ovals denote a CACCC site in the construct that Klf3 binds to in vitro, light closed ovals denote partial binding in vitro, and open ovals are CACCC sites that Klf3 does not bind in vitro.