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NEIL1 interaction with FEN-1 is essential for efficient NEIL1-initiated strand displacement repair synthesis. A, incorporation of labeled [32P]dTMP was used as a measure of strand displacement repair. B, inhibition of NEIL1-initiated LP-BER in the presence of point mutants of NEIL1 and/or FEN-1 with decreased interaction (lanes 3–7). PNK, Polβ, DNA ligase IIIα, and PCNA were present in all reactions.
